解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::As an initial screen for novel markers of renal cancer and to minimise background heterogeneity, we have compared the within-patient profiles of serum samples from seven patients pre- and post-nephrectomy. Samples were depleted of six of the most abundant proteins using Agilent's multiple affinity removal system (MARS...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800497
更新日期:2008-12-01 00:00:00
abstract::DIGE is a protein labelling and separation technique allowing quantitative proteomics of two or more samples by optical fluorescence detection of differentially labelled proteins that are electrophoretically separated on the same gel. DIGE is an alternative to quantitation by MS-based methodologies and can circumvent ...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800298
更新日期:2008-12-01 00:00:00
abstract::Elite rice hybrids, when compared to their parental lines, exhibit increased yield and other favorable agronomical traits, such as pathogen- and water-stress resistances, which are described as heterosis, and the molecular mechanism of heterosis remains to be elucidated. Since genomic sequences of the paternal (9311) ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701164
更新日期:2008-11-01 00:00:00
abstract::Chemokines (CCs) are small chemoattractant cytokines involved in a wide variety of biological and pathological processes. Released by cells in the milieu, and extracellular matrix and activating signalling cascades upon binding to specific G protein-coupled receptors (GPCRs), they trigger many cellular events. In vari...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800211
更新日期:2008-11-01 00:00:00
abstract::With recent publications of several large-scale protein-protein interaction (PPI) studies, the realization of the full yeast interaction network is getting closer. Here, we have analysed several yeast protein interaction datasets to understand their strengths and weaknesses. In particular, we investigate the effect of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800150
更新日期:2008-11-01 00:00:00
abstract::The quantitative analysis of signaling networks requires highly sensitive methods for the time-resolved determination of protein phosphorylation. For this reason, we developed a quantitative protein microarray that monitors the activation of multiple signaling pathways in parallel, and at high temporal resolution. A l...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800112
更新日期:2008-11-01 00:00:00
abstract::Production of membrane proteins (MPs) is a challenging task as their hydrophobic nature and their specific requirements in cellular expression systems frequently prevent an efficient synthesis. Cell-free (CF) expression systems have been developed in recent times as promising tools by offering completely new approache...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800171
更新日期:2008-10-01 00:00:00
abstract::Integral membrane proteins (IMPs) perform crucial cellular functions and are the primary targets for most pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains and their intimate association with lipids make them difficult to handle. Numerous proteomic platforms that include LC sepa...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200800210
更新日期:2008-10-01 00:00:00
abstract::Along with unequivocal hits produced by matching multiple MS/MS spectra to database sequences, LC-MS/MS analysis often yields a large number of hits of borderline statistical confidence. To simplify their validation, we propose to use rapid de novo interpretation of all acquired MS/MS spectra and, with the help of a s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800250
更新日期:2008-10-01 00:00:00
abstract::Myogenesis is a complex process of which the underlying mechanisms are conserved between species, including birds and mammals. Despite a good understanding of the stages of myogenesis, many of the mechanisms involved in the regulation of proliferation of the successive myoblast generations, the cellular transitions ce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701101
更新日期:2008-10-01 00:00:00
abstract::We have used MALDI-MS imaging (MALDI-MSI) to monitor the time dependent appearance and loss of signals when tissue slices are brought rapidly to room temperature for short to medium periods of time. Sections from mouse brain were cut in a cryostat microtome, placed on a MALDI target and allowed to warm to room tempera...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800201
更新日期:2008-09-01 00:00:00
abstract::After hind limb suspension, a remodeling of postural muscle phenotype is observed. This remodeling results in a shift of muscle profile from slow-oxidative to fast-glycolytic. These metabolic changes and fiber type shift increase muscle fatigability. Acetyl-L-carnitine (ALCAR) influences the skeletal muscle phenotype ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701176
更新日期:2008-09-01 00:00:00
abstract::Lectin microarray is an emerging technique, which will accelerate glycan profiling and discovery of glycan-related biomarkers. One of the most important stages in realizing the potential of the technique is to achieve sufficiently high sensitivity to detect even the low concentrations of some target glycoproteins whic...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701114
更新日期:2008-08-01 00:00:00
abstract::Many tumors arising from epithelial tissues produce mucins, which readily come into contact with infiltrating cells in cancer tissues. MUC2 mucins were purified from the conditioned medium of a colorectal cancer cell line, LS180 cells. It is known that in cancer patients, the number of dendritic cells (DCs) is reduced...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800039
更新日期:2008-08-01 00:00:00
abstract::Metabolite flux to UDP-GlcNAc and Golgi N-glycan biosynthesis regulates surface residency of glycoprotein receptors and transporters, and thus sensitivities of cells to extracellular cues. Salvage of GlcNAc increases UDP-GlcNAc and branching of N-glycans progressively, but displays an optimum for cell proliferation an...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800208
更新日期:2008-08-01 00:00:00
abstract::Most proteins in all organisms undergo crucial N-terminal modifications involving N-terminal methionine excision, N-alpha-acetylation or N-myristoylation (N-Myr), or S-palmitoylation. We investigated the occurrence of these poorly annotated but essential modifications in proteomes, focusing on eukaryotes. Experimental...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701191
更新日期:2008-07-01 00:00:00
abstract::By means of a degradomic approach applying proteomic techniques, we previously suggested that apolipoprotein E (apoE) is a substrate of matrix metalloproteinase-14 (MMP-14). Here we confirm that apoE is, in fact, a substrate of MMP-14 and also of MMP-7 and MMP-2 to a lesser extent. The 34 kDa apoE protein was initiall...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700487
更新日期:2008-07-01 00:00:00
abstract::Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by u...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200800009
更新日期:2008-07-01 00:00:00
abstract::The butyrate-producing anaerobe Fusobacterium varium is an integral constituent of human gut microflora. Unlike many gut microorganisms, F. varium is capable of fermenting both amino acids and glucose. Although F. varium has been implicated in beneficial as well as pathological bacterium-host interactions, its genome ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700437
更新日期:2008-07-01 00:00:00
abstract::Sandwich ELISA microarrays have great potential for validating disease biomarkers. Each ELISA relies on robust-affinity reagents that retain activity when immobilized on a solid surface or when labeled for detection. Single-chain antibodies (scFv) are affinity reagents that have greater potential for high-throughput p...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701036
更新日期:2008-06-01 00:00:00
abstract::The Human Kidney and Urine Proteome Project (HKUPP) was initiated in 2005 to promote proteomics research in the nephrology field, to better understand kidney functions as well as pathogenic mechanisms of kidney diseases, and to define novel biomarkers and therapeutic targets. This project was first approved in 2005 by...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200800138
更新日期:2008-06-01 00:00:00
abstract::Cadmium (Cd) pollution is a worldwide major concern having, among others, deleterious effects on plants. In the present work, the effects of a 20 microM Cd exposure in hydroponics culture during 14 days were evaluated in young poplar leaves. Proteins were analysed by 2-D DIGE, followed by MALDI-TOF-TOF identification....
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701110
更新日期:2008-06-01 00:00:00
abstract::Pathogenic bacteria excrete a variety of virulence factors into extracellular medium and to the cell surface which have essential roles in the colonization and insurrection of the host cells, and thus reflect the degree of bacterial pathogenicity. For the exploration of virulence factors expressed in the secreted prot...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700965
更新日期:2008-06-01 00:00:00
abstract::We here present a user-friendly and extremely lightweight tool that can serve as a stand-alone front-end for the Open MS Search Algorithm (OMSSA) search engine, or that can directly be used as part of an informatics processing pipeline for MS driven proteomics. The OMSSA graphical user interface (OMSSAGUI) tool is wri...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701126
更新日期:2008-06-01 00:00:00
abstract::Each postnatal hair follicle (HF) perpetually goes through three phases: anagen, catagen, and telogen. The molecular signals that orchestrate the follicular transition between phases are still largely unknown. Our previous study shows that the keratinocyte specific Smad4 knockout mice exhibit progressive alopecia due ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700760
更新日期:2008-06-01 00:00:00
abstract::The influenza virus polymerase is formed by the PB1, PB2 and PA subunits and is required for virus transcription and replication in the nucleus of infected cells. Here we present the characterisation of the complexes formed intracellularly by the influenza polymerase in human cells. The virus polymerase was expressed ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700508
更新日期:2008-05-01 00:00:00
abstract::The last years have seen the emergence of many large-scale proteomics initiatives that have identified thousands of new protein interactions and macromolecular assemblies. However, unfortunately, only a few among the discovered complexes meet the high-quality standards required to be promptly used in structural studie...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700966
更新日期:2008-05-01 00:00:00
abstract::We present a novel dataset assessing the specificity of protein-protein interactions between 69 transmitter and receiver domains from two-component system (TCS)-signalling pathways. TCS require a conserved protein-protein interaction between partner transmitter and receiver domains for signal transduction. The complex...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700544
更新日期:2008-05-01 00:00:00
abstract::When the p-value is set at <0.05 in statistical group comparisons, a 5% rate of "false significant" results is expected. In order to test the reliability of our 2-DE method, we loaded each of 24 gels with equal-sized samples (200 mug protein from pooled rat brain, pH 4-7, stained with ruthenium fluorescent stain for v...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701074
更新日期:2008-05-01 00:00:00
abstract::The plenary session of the Proteomics Standards Initiative of the Human Proteome Organisation at the 6th annual HUPO world congress updated the delegates on the current status of the ongoing work of this group. The new MS interchange format, mzML, had been entered into the HUPO document process immediately prior to th...
journal_title:Proteomics
pub_type:
doi:10.1002/pmic.200701086
更新日期:2008-04-01 00:00:00
abstract::Soil salinity is a major abiotic constraint to agricultural productivity. We successfully bred a new common wheat (Triticum aestivum L.) introgression variety (Shanrong No. 3) with high salt-tolerance via asymmetric somatic hybridization between common wheat cultivar (Jinan 177) and UV-irradiated Agropyron elongatum (...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700569
更新日期:2008-04-01 00:00:00
abstract::Ibuprofen is a member of the proprionic acid group of nonsteroidal anti-inflammatory drugs (NSAID), with the S-enantiomer being more active than the R-enantiomer. It has been shown to display protective effects against neuroinflammation, which is linked to the pathogenesis of several neurodegenerative disorders, inclu...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700556
更新日期:2008-04-01 00:00:00
abstract::This is the first broad proteomic description of Gluconacetobacter diazotrophicus, an endophytic bacterium, responsible for the major fraction of the atmospheric nitrogen fixed in sugarcane in tropical regions. Proteomic coverage of G. diazotrophicus PAL5 was obtained by two independent approaches: 2-DE followed by MA...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700912
更新日期:2008-04-01 00:00:00
abstract::In 2001, the German Federal Ministry of Education and Research (BMBF) initiated the National Genome Research Network (NGFN; www.ngfn.de) as a nation-wide multidisciplinary networking platform aiming at the analysis of common human diseases and aging. Within the NGFN the Human Brain Proteome Project (HBPP; www.smp-prot...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700980
更新日期:2008-03-01 00:00:00
abstract::Accumulation of proteins in inclusions in neurological disorders is partly due to dysfunction of the ubiquitin-proteasome system. Proteasomal dysfunction may be caused by misexpression of one or more of its subunits. A large number of antibodies reactive with proteasome subunits were screened on material from patients...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700679
更新日期:2008-03-01 00:00:00
abstract::Oligogalacturonides (OGs) are elicitors of plant defence responses released from the homogalacturonan of the plant cell wall during the attack by pathogenic micro-organisms. The signalling pathway mediated by OGs remains poorly understood, and no proteins involved in their signal perception and transduction have yet b...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700523
更新日期:2008-03-01 00:00:00
abstract::Ubiquitin-dependent proteolysis is mediated by the proteasome. To understand the structure and function of the human 26S proteasome, we cloned complete ORFs of 32 human proteasome subunits and conducted a yeast two-hybrid analysis of their interactions with each other. We observed that there are 114 interacting-pairs ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200700588
更新日期:2008-02-01 00:00:00
abstract::Sequencing of the human genome opened the way to the exploration of the proteome and this has lead to the identification of large numbers of proteins in complex biological samples. The identification of diagnostic patterns in samples taken from patients to aid diagnosis is in the early stages of development. The solut...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700665
更新日期:2008-02-01 00:00:00
abstract::Mitochondria were isolated from whole hearts of Dahl salt sensitive (SS) and chromosome 13 consomic control (SS.13BN/Mcwi) rats using a mechanical homogenization process followed by density centrifugation. The proteins present in the two mitochondria preparations were quantified; equal amounts of protein from each sam...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200701009
更新日期:2008-02-01 00:00:00
abstract::MALDI-MS is an extremely flexible technique and can be synergistically used in conjunction with established bioanalytical methods such as PAGE or SPR. To that end, slight modifications on the sample target plate may be necessary. Those can involve the use of hydrophobic coatings for improved sample deposition and desa...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200700782
更新日期:2008-02-01 00:00:00